Composite

Part:BBa_K3490001:Design

Designed by: Ryan Huang   Group: iGEM20_NCKU_Tainan   (2020-10-23)


IPTG inducible NOS, over-express csgD and csgA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3923
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1826
    Illegal SapI.rc site found at 2144


Design Notes

the strain of the bacteria needs to have a T7 promoter.



Source

Derived from IDT synthesis.

References

[1] Barnhart MM, Chapman MR. Curli Biogenesis and Function. Annual Review of Microbiology. 2006;60(1):131-147. ‌

[2] Part:BBa K805015 - parts.igem.org. Igem.org. https://parts.igem.org/Part:BBa_K805015. Published 2013. Accessed September 21, 2020.